A Modified Carbon Monoxide Breath Test for Measuring Erythrocyte…
A Modified Carbon Monoxide Breath Test for Measuring Erythrocyte Lifespan in Small Animals
Yong-JianMa,1 Hou-De Zhang,1,2 Yong-Qiang Ji,3 Guo-Liang Zhu,3 Jia-Liang Huang,3Li-Tao Du,3 Ping Cao,4 De-Yue Zang,4 Ji-Hui Du,5 Rong Li,5 and Lei Wang5
1 Institute of Breath Test Research, Shenzhen University, Shenzhen 518060, China
2Department of Gastroenterology, Nanshan Hospital, Guangdong Medical College, Shenzhen 518052, China
3Institute of Seekya Breath Test Technology, Shenzhen 518053, China
4Shenzhen Testing Center of Medical Devices, Shenzhen 518000, China
5Central Laboratory, Nanshan Hospital, Guangdong Medical College, Shenzhen 518052, China
Correspondence should be addressed to Hou-De Zhang; szkjk@126.com
Received 16 February 2016; Accepted 10 April 2016
Academic Editor: Yoshifumi Saisho
Copyright . 2016 Yong-Jian Ma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study was to develop a CO breath test for RBC lifespan estimation of small animals. The ribavirin induced hemolysis rabbit modelswere placed individually in a closed rebreath cage and air sampleswere collected formeasurement ofCOconcentration. RBC lifespan was calculated fromaccumulated CO, blood volume, and hemoglobin concentration data. RBC lifespan was determined in the same animals with the standard biotin-labeling method. RBC lifespan data obtained by the CO breath test method for control (CON, 49.0±5.9 d) rabbits, rabbits given 10mg/kg⋅d−1 of ribavirin (RIB10, 31.0±4.0 d), and rabbits given 20mg/kg⋅d−1 of ribavirin (RIB20, 25.0 ± 2.9 d) were statistically similar (all
